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LDAP Queries for user accounts and groups

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I am busy with a project were I need to restructure my current AD and decommission 2 Domain Controllers. This involves creating new OUs and moving user accounts/service accounts and groups to different OUs as well.

Is it possible to run a PS command, or any type of report to determine if any applications are using those particular DC's for LDAP queries, and also which accounts and groups are also being used? We have 100s of applications and no documentation for the setup on them. If I can get a report of which IP is talking to the DC for LDAP a query, I can use that to match my application so narrow down and get the application updated to use the new DC. I can also do the same for the accounts as well. 

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